MARK2/EMK1/Par-1Bα Phosphorylation of Rab11-Family Interacting Protein 2 Is Necessary for the Timely Establishment of Polarity in Madin-Darby Canine Kidney Cells
نویسندگان
چکیده
منابع مشابه
MARK2/EMK1/Par-1Balpha phosphorylation of Rab11-family interacting protein 2 is necessary for the timely establishment of polarity in Madin-Darby canine kidney cells.
Rab11a, myosin Vb, and the Rab11-family interacting protein 2 (FIP2) regulate plasma membrane recycling in epithelial cells. This study sought to characterize more fully Rab11-FIP2 function by identifying kinase activities modifying Rab11-FIP2. We have found that gastric microsomal membrane extracts phosphorylate Rab11-FIP2 on serine 227. We identified the kinase that phosphorylated Rab11-FIP2 ...
متن کاملCytoskeletal control of centrioles movement during the establishment of polarity in Madin-Darby canine kidney cells
The two centrioles that are localized close to each other and to the nucleus in single Madin-Darby Canine kidney cells (MDCK) move apart by distances as large as 13 microns after the establishment of extensive cellular junctions. Microfilaments, and possibly microtubules appear to be responsible for this separation. In fully polarized cells, the centrioles are localized just beneath the apical ...
متن کاملSorting of sphingolipids in epithelial (Madin-Darby canine kidney) cells
To study the intracellular transport of newly synthesized sphingolipids in epithelial cells we have used a fluorescent ceramide analog, N-6[7-nitro-2,1,3-benzoxadiazol-4-yl] aminocaproyl sphingosine (C6-NBD-ceramide; Lipsky, N. G., and R. E. Pagano, 1983, Proc. Natl. Acad. Sci. USA, 80:2608-2612) as a probe. This ceramide was readily taken up by filter-grown Madin-Darby canine kidney (MDCK) cel...
متن کاملTitle: Ca 2+ movement and apoptosis induced by deltamethrin in Madin – Darby canine kidney canine renal tubular cells Keywords: Apoptosis, Ca2+, Deltamethrin, Madin–Darby canine kidney, Renal cells
Title: Ca 2+ movement and apoptosis induced by deltamethrin in Madin – Darby canine kidney canine renal tubular cells Keywords: Alveolar bone grafting, Antigen-extracted xenogeneic cancellous bone, Osteogenesis, Recombinant human bone morphogenetic protein-2 26-33 Title: Clinical performance of a new hepatitis B surface antigen quantitative assay with automatic dilution Title: Noncontrast compu...
متن کاملPolarity of endogenous and exogenous glycosyl-phosphatidylinositol-anchored membrane proteins in Madin-Darby canine kidney cells.
Madin-Darby canine kidney cells (MDCK) were transfected with a cDNA encoding the glycosyl-phosphatidylinositol (GPI)-anchored protein mouse Thy-1 in order to study the steady-state surface distribution of exogenous and endogenous GPI-linked proteins. Immunofluorescence of transfected cells grown on collagen-coated coverslips showed that expression of Thy-1 was variable throughout the epithelium...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecular Biology of the Cell
سال: 2006
ISSN: 1059-1524,1939-4586
DOI: 10.1091/mbc.e05-08-0736